From owner-chemistry@ccl.net Sat May 15 10:58:00 2010 From: "Basma Ghazal basmaghazal%%ymail.com" To: CCL Subject: CCL: Effect of sulphate ions on the preparation of the enzyme Message-Id: <-41863-100515025436-22943-OFUNW9gYltZiQct4YWPq3A/a\server.ccl.net> X-Original-From: Basma Ghazal Content-Type: multipart/alternative; boundary="0-527745345-1273906466=:17284" Date: Fri, 14 May 2010 23:54:26 -0700 (PDT) MIME-Version: 1.0 Sent to CCL by: Basma Ghazal [basmaghazal ~ ymail.com] --0-527745345-1273906466=:17284 Content-Type: text/plain; charset=us-ascii Dear All, Thanks for your helpful suggestion. The sulphate ion is coming from the X-ray crystallization of the protein; this mention on experimental paper of extraction of this protein, so from my knowledge it is necessary to remove them from the protein. I hope to know if there are any other tricks to solve your problem rather than removing the sulfate ion. Any suggestion will be thanked, --0-527745345-1273906466=:17284 Content-Type: text/html; charset=us-ascii

Dear All,

Thanks for your helpful suggestion. The sulphate ion is coming from the X-ray crystallization of the protein; this mention on experimental paper of extraction of this protein, so from my knowledge it is necessary to remove them from the protein. I hope to know if there are any other tricks to solve your problem rather than removing the sulfate ion.

Any suggestion will be thanked,


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